Applied Bioinformatics Group


A   A   A
Sections
Home > Publications > No evidence of viral genomes in whole-transcriptome sequencings of three melanoma metastases

Skip to content. | Skip to navigation

Document Actions

Magdalena Feldhahn, Moritz Menzel, Benjamin Weide, Peter Bauer, Diana Meckbach, Claus Garbe, Oliver Kohlbacher, and J├╝rgen Bauer (2011)

No evidence of viral genomes in whole-transcriptome sequencings of three melanoma metastases

Exp. Dermatol., 20(9):766-768 .

Several viruses are known to cause cancer, such as human herpes virus 8 in Kaposi sarcoma and human papilloma viruses in cervical cancer. Recently, Merkel cell polyoma virus (MCPyV) has been described in 80% of Merkel cell carcinomas (MCC). Similarly to MCC and Kaposi sarcoma, melanoma incidence is increased in immunosuppressed patients. We asked whether infection by known or yet unknown viruses may play a role in melanoma development as well. To detect viral sequences expressed in melanoma cells, we analysed three melanoma metastases by whole-transcriptome sequencing and digital transcriptome subtraction. None of the samples investigated harboured viral sequences. In contrast, artificial viral sequences and MCPyV transcripts used as a positive control for the bioinformatics analysis were detected. This renders it less likely that viruses are frequently involved in melanoma induction. A larger number of melanoma transcriptome sequencings are required to rule out viruses as a relevant pathogen.
21672032
10.1111/j.1600-0625.2011.01312.x